Hotspot

trnN-GUU-ndhF

IRb · 111433-112982 · 1550 bp

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Pi 0.00151
MarkerSeek score 54.30
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1550
variable_sites
4
pi
0.00151
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 AGGATTCGAACCTACGACCA CAAATATGTAGGGGGCGGAC 50.0 55.0 58.2 58.1 1659-1709 bp 1.0 80.7
trnN-GUU-ndhF_p2 AAAGCGAGCCCTTTCATTCT CAAATATGTAGGGGGCGGAC 45.0 55.0 57.8 58.1 1717-1767 bp 1.0 80.2
trnN-GUU-ndhF_p3 AGGATTCGAACCTACGACCA TCAAATATGTAGGGGGCGGA 50.0 50.0 58.2 58.2 1660-1710 bp 1.0 80.0
trnN-GUU-ndhF_p4 AATCAGTTAACAGCCGACCG CAAATATGTAGGGGGCGGAC 50.0 55.0 58.3 58.1 1640-1690 bp 1.0 79.7
trnN-GUU-ndhF_p5 AAAGCGAGCCCTTTCATTCT TCAAATATGTAGGGGGCGGA 45.0 50.0 57.8 58.2 1718-1768 bp 1.0 79.5