Hotspot

trnF-GAA-ndhJ

LSC · 50428-51076 · 649 bp

Back to Akebia

Pi 0.00309
MarkerSeek score 52.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
649
variable_sites
4
pi
0.00309
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA TGCCTGAAGGTTGGATAGGA 55.0 50.0 58.0 58.0 798-801 bp 1.0 80.4
trnF-GAA-ndhJ_p2 ATGATGCATCGGGAAAGGTC TGCCTGAAGGTTGGATAGGA 50.0 50.0 58.0 58.0 818-821 bp 1.0 80.2
trnF-GAA-ndhJ_p3 GATGATGCATCGGGAAAGGT TGCCTGAAGGTTGGATAGGA 50.0 50.0 58.0 58.0 819-822 bp 1.0 80.2
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA TGCCTGAAGGTTGGATAGGA 50.0 50.0 58.1 58.0 762-765 bp 1.0 80.0
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA AGGTTGGATAGGATGGCCTT 55.0 50.0 58.0 58.1 791-794 bp 1.0 80.0