Hotspot

ycf3-trnS-GGA

LSC · 46258-47227 · 970 bp

Back to Akebia

Pi 0.00533
MarkerSeek score 64.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
970
variable_sites
9
pi
0.00533
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CCATTTATCTGCGATCTAGGCA AACGTACGGAAAGAGAGGGA 45.5 50.0 58.1 58.1 1036-1487 bp 1.0 67.1
ycf3-trnS-GGA_p2 CCATTTATCTGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 45.5 50.0 58.1 58.1 1010-1461 bp 1.0 67.1
ycf3-trnS-GGA_p3 CCATTTATCTGCGATCTAGGCA ACGTACGGAAAGAGAGGGAT 45.5 50.0 58.1 57.9 1035-1486 bp 1.0 66.7
ycf3-trnS-GGA_p4 CCATTTATCTGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 45.5 50.0 58.1 58.2 1031-1482 bp 1.0 66.6
ycf3-trnS-GGA_p5 CCATTTATCTGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 45.5 45.0 58.1 57.8 981-1432 bp 1.0 66.5