Hotspot

psbC-trnS-UGA

LSC · 37248-37445 · 198 bp

Back to Akebia

Pi 0.0429
MarkerSeek score 68.50
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
198
variable_sites
17
pi
0.0429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 AGCTGCAGCAGGATTTGAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.4 58.1 348-349 bp 1.0 80.4
psbC-trnS-UGA_p2 GCTGCAGCAGGATTTGAAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.6 58.1 347-348 bp 1.0 79.9
psbC-trnS-UGA_p3 AGCTGCAGCAGGATTTGAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 58.4 58.2 294-295 bp 1.0 79.9
psbC-trnS-UGA_p4 AGCTGCAGCAGGATTTGAAA GATGGCCGAGTGGTTGATAG 45.0 55.0 58.4 58.1 359-360 bp 1.0 79.7
psbC-trnS-UGA_p5 GCTGCAGCAGGATTTGAAAA GATGGCCGAGTGGTTGATAG 45.0 55.0 57.6 58.1 358-359 bp 1.0 79.2