Hotspot

trnH-GUG-psbA

LSC · 103-579 · 477 bp

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Pi 0.00943
MarkerSeek score 50.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
477
variable_sites
9
pi
0.00943
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CCACTTGGCTACATCCGC GCTCCAGCGACAAATGGATA 61.1 50.0 58.2 58.0 511-520 bp 1.0 64.6
trnH-GUG-psbA_p2 CCACTTGGCTACATCCGC CCTCTAGACCTAGCTGCTGT 61.1 55.0 58.2 57.9 535-544 bp 1.0 64.3
trnH-GUG-psbA_p3 CCACTTGGCTACATCCGC TTCCCTCTAGACCTAGCTGC 61.1 55.0 58.2 57.9 538-547 bp 1.0 64.3
trnH-GUG-psbA_p4 CCACTTGGCTACATCCGC TAGACCTAGCTGCTGTCGAA 61.1 50.0 58.2 57.9 531-540 bp 1.0 63.9
trnH-GUG-psbA_p5 CCACTTGGCTACATCCGC CTAGACCTAGCTGCTGTCGA 61.1 55.0 58.2 58.3 532-541 bp 1.0 62.2