Hotspot

ycf1

SSC · 120455-125881 · 5427 bp

Back to Ajuga

Pi 0.00863
MarkerSeek score 49.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5427
variable_sites
245
pi
0.00863
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGGCCCAGTATTCTCCTTGT GAACGGGTCGTATCGGAAAA 50.0 50.0 58.0 58.0 448-472 bp 0.866667 78.8
ycf1_p2 TTTACGCCTTTGAGATCGCA GAACGGGTCGTATCGGAAAA 45.0 50.0 57.9 58.0 562-586 bp 0.866667 77.7
ycf1_p3 GCGCAAATGGAATGTTCTCC GAACGGGTCGTATCGGAAAA 50.0 50.0 58.1 58.0 824-848 bp 0.8 74.6
ycf1_p4 AGGCCCAGTATTCTCCTTGT GATCCTTTCCTGAACGGGTC 50.0 55.0 58.0 58.0 459-564 bp 0.533333 65.1
ycf1_p5 GCGCAAATGGAATGTTCTCC GATCCTTTCCTGAACGGGTC 50.0 55.0 58.1 58.0 835-940 bp 0.533333 63.5