Hotspot

rpl16

LSC · 79313-80549 · 1237 bp

Back to Ajuga

Pi 0.00766
MarkerSeek score 53.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1237
variable_sites
37
pi
0.00766
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 GGATTGCGCCAAAAATTCGA CAAGTACAAATTGCAGGGCG 45.0 50.0 58.0 58.0 1983-2032 bp 1.0 83.1
rpl16_p2 GCTTCTTTGATCACCGCAAC CAAGTACAAATTGCAGGGCG 50.0 50.0 58.0 58.0 1813-1862 bp 1.0 83.1
rpl16_p3 TGCGCCAAAAATTCGAGTTC CAAGTACAAATTGCAGGGCG 45.0 50.0 58.0 58.0 1979-2028 bp 1.0 82.9
rpl16_p4 CACCGCAACAATAACGTCAC CAAGTACAAATTGCAGGGCG 50.0 50.0 58.1 58.0 1802-1851 bp 1.0 82.9
rpl16_p5 ACCGCTGTCACGTTTAAGTT CAAGTACAAATTGCAGGGCG 45.0 50.0 58.1 58.0 1898-1919 bp 0.933333 79.3