Hotspot

ndhE

SSC · 114466-114771 · 306 bp

Pi 0.00261

MarkerSeek score 22.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 3
pi: 0.00261
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TACATCCGTCCCAGGGTATC	ACGTCCTGGTATGGGATCAT	55.0	50.0	58.0	57.9	1057-1086 bp	1.0	85.7
ndhE_p2	CCTTGCCCGTATCAAATCCA	TCTTTCTGGGATCGGGTCTT	50.0	50.0	57.9	58.0	1089-1118 bp	1.0	85.6
ndhE_p3	ACCTTGCCCGTATCAAATCC	TCTTTCTGGGATCGGGTCTT	50.0	50.0	57.9	58.0	1090-1119 bp	1.0	85.5
ndhE_p4	TACATCCGTCCCAGGGTATC	CGTATTTTGTAGCTGCTGCG	55.0	50.0	58.0	57.9	1245-1274 bp	1.0	85.2
ndhE_p5	TACATCCGTCCCAGGGTATC	TCTTTCTGGGATCGGGTCTT	55.0	50.0	58.0	58.0	1371-1400 bp	1.0	85.0