Hotspot

ccsA-ndhD

SSC · 112087-112340 · 254 bp

Back to Ajuga

Pi 0.0254
MarkerSeek score 66.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
254
variable_sites
23
pi
0.0254
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CAGATTCAGCAATTGTGGCG ACTCGGTATTGGTATGTACCCT 50.0 45.5 58.1 58.1 347-464 bp 0.933333 66.8
ccsA-ndhD_p2 GATTCAGCAATTGTGGCGTC ACTCGGTATTGGTATGTACCCT 50.0 45.5 58.1 58.1 345-462 bp 0.933333 66.8
ccsA-ndhD_p3 CAGATTCAGCAATTGTGGCG TCGGTATTGGTATGTACCCTGA 50.0 45.5 58.1 58.1 345-462 bp 0.933333 66.8
ccsA-ndhD_p4 GATTCAGCAATTGTGGCGTC TCGGTATTGGTATGTACCCTGA 50.0 45.5 58.1 58.1 343-460 bp 0.933333 66.8
ccsA-ndhD_p5 TTCAGCAATTGTGGCGTCTA ACTCGGTATTGGTATGTACCCT 45.0 45.5 57.8 58.1 343-460 bp 0.933333 66.2