Hotspot

trnL-UAG-ccsA

SSC · 111022-111114 · 93 bp

Back to Ajuga

Pi 0.0225
MarkerSeek score 67.50
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
93
variable_sites
9
pi
0.0225
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TGGAAACGGTCGAAAAAGAA 50.0 40.0 57.9 55.8 205-216 bp 1.0 68.4
trnL-UAG-ccsA_p2 GGTAGACACGCTGCTCTTAG TGGAAACGGTCGAAAAAGAA 55.0 40.0 57.8 55.8 207-218 bp 1.0 67.8
trnL-UAG-ccsA_p3 GTAGACACGCTGCTCTTAGG TGGAAACGGTCGAAAAAGAA 55.0 40.0 57.8 55.8 206-217 bp 1.0 67.8
trnL-UAG-ccsA_p4 AAATTGGTAGACACGCTGCT TGGAAACGGTCGAAAAAGAA 45.0 40.0 57.8 55.8 212-223 bp 1.0 67.7
trnL-UAG-ccsA_p5 AATTGGTAGACACGCTGCTC TGGAAACGGTCGAAAAAGAA 50.0 40.0 58.3 55.8 211-222 bp 1.0 67.1