Hotspot

ndhF-rpl32

SSC · 109830-110365 · 536 bp

Back to Ajuga

Pi 0.0155
MarkerSeek score 62.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
536
variable_sites
39
pi
0.0155
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GAAAGGCAAAATCCACGCAT AGGAAAAGGCCTTTAACGCT 45.0 45.0 57.6 57.7 664-721 bp 1.0 82.4
ndhF-rpl32_p2 GAAAGGCAAAATCCACGCAT CCCCAAGGAAAAGGCCTTTA 45.0 50.0 57.6 57.7 669-726 bp 1.0 82.1
ndhF-rpl32_p3 GAAAGGCAAAATCCACGCAT CTTTAACGCTGCCCAATGTC 45.0 50.0 57.6 57.7 654-711 bp 0.933333 77.3
ndhF-rpl32_p4 GAAAGGCAAAATCCACGCAT GAAAAGGCCTTTAACGCTGC 45.0 50.0 57.6 58.3 662-719 bp 0.933333 77.0
ndhF-rpl32_p5 AACGAAAGGCAAAATCCACG CTTTAACGCTGCCCAATGTC 45.0 50.0 57.6 57.7 657-714 bp 0.933333 76.9