Hotspot

petA-psbJ

LSC · 61602-62494 · 893 bp

Back to Ajuga

Pi 0.0102
MarkerSeek score 63.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
893
variable_sites
39
pi
0.0102
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA TTCCGGATTAGGCTCATCCC 50.0 55.0 58.2 58.9 942-968 bp 1.0 75.7
petA-psbJ_p2 TCGAGAAGGTTCAATTGGCC TTCCGGATTAGGCTCATCCC 50.0 55.0 58.2 58.9 944-970 bp 1.0 75.7
petA-psbJ_p3 GAGAAGGTTCAATTGGCCGA CGGATTAGGCTCATCCCTGT 50.0 55.0 58.2 58.9 939-965 bp 1.0 75.7
petA-psbJ_p4 TCGAGAAGGTTCAATTGGCC CGGATTAGGCTCATCCCTGT 50.0 55.0 58.2 58.9 941-967 bp 1.0 75.7
petA-psbJ_p5 CCCATTACGTGTCCAAGGTC TTCCGGATTAGGCTCATCCC 55.0 55.0 58.3 58.9 1027-1053 bp 1.0 74.7