Hotspot

rps2-rpoC2

LSC · 16356-16590 · 235 bp

Back to Ajuga

Pi 0.0216
MarkerSeek score 61.50
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
235
variable_sites
17
pi
0.0216
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CCTCCAGCATCTCTTCCAAG GGGGGAAAATGAGAGATATTTTGT 55.0 37.5 57.7 57.0 344-365 bp 1.0 48.5
rps2-rpoC2_p2 CCTCCAGCATCTCTTCCAAG TGTTTCACCACAGAAAATTCTTTGA 55.0 32.0 57.7 58.1 323-344 bp 1.0 48.3
rps2-rpoC2_p3 AAATGAACTCCTGCCTCCAG GGGGGAAAATGAGAGATATTTTGT 50.0 37.5 57.5 57.0 357-378 bp 1.0 48.3
rps2-rpoC2_p4 CCTCCAGCATCTCTTCCAAG TGTTTCACCACAGAAAATTCT 55.0 33.3 57.7 53.8 337 bp 0.066667 2.7
rps2-rpoC2_p5 CCTCCAGCATCTCTTCCAAG TGTTTCACCACAGAAAATTCTTTG 55.0 33.3 57.7 56.8 337 bp 0.066667 2.7