Hotspot

atpF-atpH

LSC · 13115-13471 · 357 bp

Back to Ajuga

Pi 0.0198
MarkerSeek score 53.90
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
357
variable_sites
21
pi
0.0198
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 GAAAGAATCGGTTACATTTTTCA GGTTGGTTGTAGCACTAGCA 30.4 50.0 53.9 57.8 410-430 bp 1.0 48.0
atpF-atpH_p2 AAAGAATCGGTTACATTTTTCA GGTTGGTTGTAGCACTAGCA 27.3 50.0 52.6 57.8 409-429 bp 1.0 48.0
atpF-atpH_p3 GAAAGAATCGGTTACATTTTTCA GGGTTGGTTGTAGCACTAGC 30.4 55.0 53.9 58.5 411-431 bp 1.0 48.0
atpF-atpH_p4 GAAAGAATCGGTTACATTTTTCAT GGTTGGTTGTAGCACTAGCA 29.2 50.0 54.5 57.8 410-430 bp 1.0 48.0
atpF-atpH_p5 GAAAGAATCGGTTACATTTTTCA CCTGAGGCAGAGGGAAAAAT 30.4 50.0 53.9 57.5 483-503 bp 1.0 47.1