Hotspot

trnS-GCU-trnG-UCC

LSC · 8423-9122 · 700 bp

Back to Ajuga

Pi 0.0109
MarkerSeek score 66.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
700
variable_sites
31
pi
0.0109
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA CGCAAGGCCCTTTAACTTTA 50.0 45.0 58.0 56.3 638-802 bp 1.0 73.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC CGCAAGGCCCTTTAACTTTA 50.0 45.0 58.0 56.3 637-801 bp 1.0 73.9
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA CGCAAGGCCCTTTAACTTTA 50.0 45.0 58.2 56.3 692-856 bp 1.0 72.5
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA CGCAAGGCCCTTTAACTTTAA 50.0 42.9 58.0 57.1 638-802 bp 1.0 71.9
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC CGCAAGGCCCTTTAACTTTAA 50.0 42.9 58.0 57.1 637-801 bp 1.0 71.9