Hotspot

ycf1

SSC · 121620-125333 · 3714 bp

Back to Ajania

Pi 0.00354
MarkerSeek score 36.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
3714
variable_sites
35
pi
0.00354
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCTTTTCTGTTTGAGTTTCACT ATCCTTTTGGTCCTCCTCGA 34.8 50.0 56.5 58.0 238 bp 1.0 45.0
ycf1_p2 TCCTTTTCTGTTTGAGTTTCACT TGGTCCTCCTCGAAAAACAC 34.8 50.0 56.5 57.8 231 bp 1.0 43.4
ycf1_p3 TCCTTTTCTGTTTGAGTTTCACT TCCTTTTGGTCCTCCTCGAA 34.8 50.0 56.5 58.3 237 bp 1.0 43.2
ycf1_p4 TCCTTTTCTGTTTGAGTTTCACTT ATCCTTTTGGTCCTCCTCGA 33.3 50.0 57.2 58.0 238 bp 1.0 42.3
ycf1_p5 CCTTTTCTGTTTGAGTTTCACTTG ATCCTTTTGGTCCTCCTCGA 37.5 50.0 57.1 58.0 237 bp 1.0 42.0