Hotspot

ndhD

SSC · 113431-114930 · 1500 bp

Pi 0.000667

MarkerSeek score 33.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 3
pi: 0.000667
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGGTTCTCGCAAAGACCAA	GCGTATCTTGTCTTTACCACG	52.6	47.6	58.4	57.1	1632 bp	1.0	53.8
ndhD_p2	GCAAAGACCAAGCGGATTG	GCGTATCTTGTCTTTACCACG	52.6	47.6	57.6	57.1	1624 bp	1.0	53.7
ndhD_p3	CGGTTCTCGCAAAGACCAAG	GCGTATCTTGTCTTTACCACG	55.0	47.6	59.5	57.1	1632 bp	1.0	53.0
ndhD_p4	GTTCTCGCAAAGACCAAGC	GCGTATCTTGTCTTTACCACG	52.6	47.6	57.2	57.1	1630 bp	1.0	50.9
ndhD_p5	GGTTCTCGCAAAGACCAAGC	GCGTATCTTGTCTTTACCACG	55.0	47.6	59.8	57.1	1631 bp	1.0	50.8