Hotspot

clpP

LSC · 68699-70695 · 1997 bp

Back to Ajania

Pi 0.00207
MarkerSeek score 54.60
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
1997
variable_sites
10
pi
0.00207
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 AAACTCGGCAGGGTGAATTT GGCTTTGTTACGTTTCCACA 45.0 45.0 58.0 56.9 2111-2121 bp 1.0 72.1
clpP_p2 AACTCGGCAGGGTGAATTTT GGCTTTGTTACGTTTCCACA 45.0 45.0 58.0 56.9 2110-2120 bp 1.0 72.1
clpP_p3 AAACTCGGCAGGGTGAATTT AGGCTTTGTTACGTTTCCACA 45.0 42.9 58.0 58.4 2112-2122 bp 1.0 70.4
clpP_p4 AACTCGGCAGGGTGAATTTT AGGCTTTGTTACGTTTCCACA 45.0 42.9 58.0 58.4 2111-2121 bp 1.0 70.4
clpP_p5 AACTCGGCAGGGTGAATTTT AGGCTTTGTTACGTTTCCAC 45.0 45.0 58.0 56.6 2111-2121 bp 1.0 69.8