Hotspot

ycf1-trnN-GUU

IRa · 125334-126989 · 1656 bp

Back to Ajania

Pi 0.00238
MarkerSeek score 53.00
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
1656
variable_sites
10
pi
0.00238
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 TTCTTCCACGATACATCCCA GAGTATCCCGGACCTACACC 45.0 60.0 55.9 58.7 1780-1813 bp 1.0 60.1
ycf1-trnN-GUU_p2 TTCTTCCACGATACATCCCA GGGGTGTTGACGGTGATTTT 45.0 50.0 55.9 58.7 1760-1793 bp 1.0 60.1
ycf1-trnN-GUU_p3 CATTCTTCCACGATACATCCCA GAGTATCCCGGACCTACACC 45.5 60.0 57.9 58.7 1782-1815 bp 1.0 59.9
ycf1-trnN-GUU_p4 CATTCTTCCACGATACATCCCA GGGGTGTTGACGGTGATTTT 45.5 50.0 57.9 58.7 1762-1795 bp 1.0 59.9
ycf1-trnN-GUU_p5 TTCTTCCACGATACATCCCA GGGTGTTGACGGTGATTTTC 45.0 50.0 55.9 57.3 1759-1792 bp 1.0 59.9