Hotspot

ndhF-rpl32

SSC · 110033-111021 · 989 bp

Back to Ajania

Pi 0.00385
MarkerSeek score 60.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
989
variable_sites
10
pi
0.00385
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTTTTTGTTGCCGTCGGAAA ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.8 57.9 1154-1193 bp 1.0 81.5
ndhF-rpl32_p2 CTTTTTGTTGCCGTCGGAAA AATGCCGCCCAATATCCTTT 45.0 45.0 57.8 57.9 1143-1182 bp 1.0 81.5
ndhF-rpl32_p3 AAGACTTTTTGTTGCCGTCG ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1158-1197 bp 1.0 79.3
ndhF-rpl32_p4 ACGAAGACTTTTTGTTGCCG ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1161-1200 bp 1.0 79.3
ndhF-rpl32_p5 CGAAGACTTTTTGTTGCCGT ACAAAGCTTTTAATGCCGCC 45.0 45.0 57.5 57.9 1160-1199 bp 1.0 79.3