Hotspot

ycf1-ndhF

SSC · 107804-107809 · 6 bp

Back to Ajania

Pi 0.111
MarkerSeek score 56.90
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
6
variable_sites
2
pi
0.111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 CTAGCACTGGGTAGACCTCA AAATGAACAAGAGTGGCCGT 55.0 45.0 57.9 58.0 1085-1125 bp 1.0 80.3
ycf1-ndhF_p2 TCGTTGTGGTCGGACTCTAT AAATGAACAAGAGTGGCCGT 50.0 45.0 58.2 58.0 1264-1304 bp 1.0 79.9
ycf1-ndhF_p3 CTAGCACTGGGTAGACCTCA GGGGATGAGAAAATTGGCCA 55.0 50.0 57.9 58.1 553-593 bp 1.0 79.9
ycf1-ndhF_p4 ATCAATTCGGTCGTTGTGGT AAATGAACAAGAGTGGCCGT 45.0 45.0 57.8 58.0 1274-1314 bp 1.0 79.8
ycf1-ndhF_p5 TCTCTTCCTTCTCCGAGCTC AAATGAACAAGAGTGGCCGT 55.0 45.0 58.2 58.0 1206-1246 bp 1.0 79.4