Hotspot

psbE-petL

LSC · 63434-64691 · 1258 bp

Back to Ajania

Pi 0.00461
MarkerSeek score 59.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1258
variable_sites
12
pi
0.00461
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.9 58.0 1387-1430 bp 1.0 81.2
psbE-petL_p2 CAACCCGCAATGAATAGGGA AAGCCGCTAGTAGAAAACCG 50.0 50.0 57.9 58.0 1389-1432 bp 1.0 81.1
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.8 58.0 1310-1353 bp 1.0 80.4
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC AAGCCGCTAGTAGAAAACCG 50.0 50.0 57.8 58.0 1312-1355 bp 1.0 80.4
psbE-petL_p5 ACGAATAACCAACCCGCAAT GCCGCTAGTAGAAAACCGAA 45.0 50.0 58.2 58.0 1396-1439 bp 1.0 80.2