Hotspot

petA-psbJ

LSC · 61898-62642 · 745 bp

Back to Ajania

Pi 0.00523
MarkerSeek score 51.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
745
variable_sites
11
pi
0.00523
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGTCCGA ATTCCGGATTGGGTTCATCC 45.0 50.0 55.4 57.6 793-804 bp 1.0 58.6
petA-psbJ_p2 GAGAAGGTTCAATTGTCCGAA ATTCCGGATTGGGTTCATCC 42.9 50.0 56.2 57.6 793-804 bp 1.0 56.9
petA-psbJ_p3 GAGAAGGTTCAATTGTCCGA TGTGATCGGTTTAGTAGGCG 45.0 50.0 55.4 57.4 831-842 bp 1.0 56.8
petA-psbJ_p4 GAGAAGGTTCAATTGTCCGA GTGATCGGTTTAGTAGGCGT 45.0 50.0 55.4 57.4 830-841 bp 1.0 56.8
petA-psbJ_p5 GAGAAGGTTCAATTGTCCGAA TGTGATCGGTTTAGTAGGCG 42.9 50.0 56.2 57.4 831-842 bp 1.0 55.1