Hotspot

atpB-rbcL

LSC · 54024-54751 · 728 bp

Back to Ajania

Pi 0.00229
MarkerSeek score 54.90
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
728
variable_sites
5
pi
0.00229
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AACCCCAGAACTAGCAGGAT ACCCCAGCTTTGAATCCAAC 50.0 50.0 58.0 58.4 802-812 bp 1.0 77.6
atpB-rbcL_p2 ACCCCAGAACTAGCAGGATT ACCCCAGCTTTGAATCCAAC 50.0 50.0 58.0 58.4 801-811 bp 1.0 77.6
atpB-rbcL_p3 AACCCCAGAACTAGCAGGAT CCCCAGCTTTGAATCCAACA 50.0 50.0 58.0 58.4 801-811 bp 1.0 77.6
atpB-rbcL_p4 ACCCCAGAACTAGCAGGATT CCCCAGCTTTGAATCCAACA 50.0 50.0 58.0 58.4 800-810 bp 1.0 77.6
atpB-rbcL_p5 CTGGCGGAAAAGCTACATCT ACCCCAGCTTTGAATCCAAC 50.0 50.0 58.0 58.4 872-882 bp 1.0 77.6