Hotspot

trnT-UGU-trnL-UAA

LSC · 46075-46576 · 502 bp

Back to Ajania

Pi 0.00876
MarkerSeek score 51.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
502
variable_sites
8
pi
0.00876
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG GCGTCTACCTATTTCGCCAT 55.0 50.0 57.9 57.8 558-567 bp 1.0 79.2
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT GCGTCTACCTATTTCGCCAT 50.0 50.0 57.8 57.8 590-599 bp 1.0 78.2
trnT-UGU-trnL-UAA_p3 ATGCGATGCTCTAACCTCTG GCGTCTACCTATTTCGCCAT 50.0 50.0 57.5 57.8 560-569 bp 1.0 76.0
trnT-UGU-trnL-UAA_p4 TGCGATGCTCTAACCTCTGA GCGTCTACCTATTTCGCCAT 50.0 50.0 58.5 57.8 559-568 bp 1.0 76.0
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG AAGTCCGTAGCGTCTACCTA 55.0 50.0 57.9 57.3 567-576 bp 1.0 74.7