Hotspot

psaA-ycf3

LSC · 41128-41857 · 730 bp

Back to Ajania

Pi 0.00553
MarkerSeek score 65.00
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
730
variable_sites
10
pi
0.00553
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GAGAAATGACCCGGTCTAGC AGCCGCTGAGTATTGGAAAC 55.0 50.0 57.8 58.3 916-929 bp 1.0 78.1
psaA-ycf3_p2 GAGAAATGACCCGGTCTAGC CAAGCCGCTGAGTATTGGAA 55.0 50.0 57.8 58.3 918-931 bp 1.0 78.1
psaA-ycf3_p3 GGGCCTTTCGCTATTGTTCT AGCCGCTGAGTATTGGAAAC 50.0 50.0 58.2 58.3 937-950 bp 1.0 77.9
psaA-ycf3_p4 GGGCCTTTCGCTATTGTTCT CAAGCCGCTGAGTATTGGAA 50.0 50.0 58.2 58.3 939-952 bp 1.0 77.9
psaA-ycf3_p5 GAGAAATGACCCGGTCTAGC AAGCCGCTGAGTATTGGAAA 55.0 45.0 57.8 57.5 917-930 bp 1.0 76.3