Hotspot

trnT-GGU-psbD

LSC · 30943-32151 · 1209 bp

Back to Ajania

Pi 0.00343
MarkerSeek score 57.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1209
variable_sites
9
pi
0.00343
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGACCCGTTCAGGAATTCA GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1373-1399 bp 1.0 80.6
trnT-GGU-psbD_p2 CAGTGACCCGTTCAGGAATT GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1375-1401 bp 1.0 80.5
trnT-GGU-psbD_p3 TAATCCCACGGTAAGGCGTA GAAACGGTCCCTACGTAACC 50.0 55.0 58.2 58.0 1316-1342 bp 1.0 80.3
trnT-GGU-psbD_p4 GAGTAATCCCACGGTAAGGC GAAACGGTCCCTACGTAACC 55.0 55.0 57.8 58.0 1319-1345 bp 1.0 80.2
trnT-GGU-psbD_p5 CGTAAGCCCTCGGTTCAAAT GAAACGGTCCCTACGTAACC 50.0 55.0 58.3 58.0 1300-1326 bp 1.0 80.0