Hotspot

trnK-UUU-rps16

LSC · 4373-5219 · 847 bp

Back to Ajania

Pi 0.00315
MarkerSeek score 55.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
847
variable_sites
8
pi
0.00315
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC GGTGCTCAACCTACAGGAAC 55.0 55.0 57.9 58.2 1001-1002 bp 1.0 78.6
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC GGTGCTCAACCTACAGGAAC 55.0 55.0 57.9 58.2 1000-1001 bp 1.0 78.6
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 940-941 bp 1.0 77.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 939-940 bp 1.0 77.9
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.8 58.2 1025-1026 bp 1.0 77.3