Hotspot

rps19

Genome · 96418-96447 · 30 bp

Back to Airampoa

Pi 0.037

MarkerSeek score 69.30

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: Genome
length_bp: 30
variable_sites: 1
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTCGCCCACCAACTACAAAA	TGGCTCGGCATGTTCTATTC	45.0	50.0	57.9	58.0	130 bp	0.5	59.2
rps19_p2	GTCAAGGCAGTGGATTGTGA	TGGCTCGGCATGTTCTATTC	50.0	50.0	58.1	58.0	180 bp	0.5	58.8
rps19_p3	TTCGCCCACCAACTACAAAA	AAGAAGATTTGGCTCGGCAT	45.0	45.0	57.9	57.9	139 bp	0.5	58.5
rps19_p4	TTCGCCCACCAACTACAAAA	AGAAGAACCCGACTCGGTAT	45.0	50.0	57.9	57.9	98 bp	0.5	58.4
rps19_p5	TTCGCCCACCAACTACAAAA	GCTCGGCATGTTCTATTCGA	45.0	50.0	57.9	58.1	128 bp	0.5	58.4