Hotspot

atpF

Genome · 24988-26288 · 1301 bp

Back to Airampoa

Pi 0.00385

MarkerSeek score 61.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 1301
variable_sites: 5
pi: 0.00385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGGGTTGCCATGAGTCTTTC	AGTAGCAAAGAGGTGAAGCA	50.0	45.0	57.8	56.8	1439-1441 bp	1.0	68.9
atpF_p2	GAAGGGTTGCCATGAGTCTT	AGTAGCAAAGAGGTGAAGCA	50.0	45.0	57.8	56.8	1441-1443 bp	1.0	68.9
atpF_p3	GGGTTGCCATGAGTCTTTCT	AGTAGCAAAGAGGTGAAGCA	50.0	45.0	57.8	56.8	1438-1440 bp	1.0	68.9
atpF_p4	GGGTTGCCATGAGTCTTTCT	AAGTAGCAAAGAGGTGAAGCA	50.0	42.9	57.8	57.5	1439-1441 bp	1.0	66.9
atpF_p5	GGGTTGCCATGAGTCTTTCT	AGTAGCAAAGAGGTGAAGCAA	50.0	42.9	57.8	57.5	1438-1440 bp	1.0	66.9