Hotspot

accD

Genome · 72437-75862 · 3426 bp

Back to Airampoa

Pi 0.0073

MarkerSeek score 45.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 3426
variable_sites: 25
pi: 0.0073
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CGCGGCTTCTAGTGATTCTT	CACCTACTACGGCTCCCATA	50.0	55.0	58.1	58.0	710-785 bp	1.0	81.0
accD_p2	ACGAGTTGAGAGAGGCTCTT	TTTTCGGTTGTTTGTGCACC	50.0	45.0	58.1	58.0	862-937 bp	1.0	80.6
accD_p3	CGCGGCTTCTAGTGATTCTT	TTTTCGGTTGTTTGTGCACC	50.0	45.0	58.1	58.0	1053-1128 bp	1.0	80.5
accD_p4	GATCGGGATGAGGATTGTCG	CACCTACTACGGCTCCCATA	55.0	55.0	57.9	58.0	1018-1093 bp	1.0	80.5
accD_p5	GATCGGGATGAGGATTGTCG	TTTTCGGTTGTTTGTGCACC	55.0	45.0	57.9	58.0	1361-1436 bp	1.0	80.3