Hotspot

trnL-UAG-ndhG

Genome · 123013-123264 · 252 bp

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Pi 0.0192

MarkerSeek score 46.10

Primer available yes

Label trnL-UAG-ndhG

Feature Metadata

label_name: trnL-UAG-ndhG
feature_type: igs
region: Genome
length_bp: 252
variable_sites: 2
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.41
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ndhG_p1	CTCGCACTGCTTCCTAAGAG	TAGGTGCAATTTCTGTGGCT	55.0	45.0	58.1	57.4	331 bp	0.5	54.7
trnL-UAG-ndhG_p2	CTCTCGCACTGCTTCCTAAG	TAGGTGCAATTTCTGTGGCT	55.0	45.0	58.1	57.4	333 bp	0.5	54.7
trnL-UAG-ndhG_p3	TCCTAAGAGCAGCGTGTCTA	TAGGTGCAATTTCTGTGGCT	50.0	45.0	57.9	57.4	320 bp	0.5	54.3
trnL-UAG-ndhG_p4	AGCAGCGTGTCTACCAATTT	TAGGTGCAATTTCTGTGGCT	45.0	45.0	57.8	57.4	313 bp	0.5	53.8
trnL-UAG-ndhG_p5	CTAAGAGCAGCGTGTCTACC	TAGGTGCAATTTCTGTGGCT	55.0	45.0	57.8	57.4	318 bp	0.5	53.8