Hotspot

ndhE-psaC

Genome · 119428-119745 · 318 bp

Back to Airampoa

Pi 0.0128

MarkerSeek score 49.60

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: Genome
length_bp: 318
variable_sites: 4
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	TGAAGCAGCTATTGGACTGG	TCATAAATCTTTACTGAATGTGACA	50.0	28.0	57.6	54.8	430-435 bp	1.0	41.7
ndhE-psaC_p2	AGCAGCTATTGGACTGGCTA	TCATAAATCTTTACTGAATGTGACA	50.0	28.0	58.5	54.8	427-432 bp	1.0	41.7
ndhE-psaC_p3	GCAGCTATTGGACTGGCTAT	TCATAAATCTTTACTGAATGTGACA	50.0	28.0	57.4	54.8	426-431 bp	1.0	41.7
ndhE-psaC_p4	TGAAGCAGCTATTGGACTGG	CATAAATCTTTACTGAATGTGACA	50.0	29.2	57.6	53.4	429-434 bp	1.0	41.7
ndhE-psaC_p5	AGCAGCTATTGGACTGGCTA	CATAAATCTTTACTGAATGTGACA	50.0	29.2	58.5	53.4	426-431 bp	1.0	41.7