Hotspot

psbA-trnH-GUG

Genome · 95929-96327 · 399 bp

Back to Airampoa

Pi 0.0101

MarkerSeek score 54.20

Primer available yes

Label psbA-trnH-GUG

Feature Metadata

label_name: psbA-trnH-GUG
feature_type: igs
region: Genome
length_bp: 399
variable_sites: 4
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH-GUG_p1	CTAGACCTAGCCGCTATCGA	TTTTGTAGTTGGTGGGCGAA	55.0	45.0	57.9	57.9	529 bp	1.0	79.6
psbA-trnH-GUG_p2	TCTAGACCTAGCCGCTATCG	TTTTGTAGTTGGTGGGCGAA	55.0	45.0	57.9	57.9	530 bp	1.0	79.6
psbA-trnH-GUG_p3	CTAGACCTAGCCGCTATCGA	TCACAATCCACTGCCTTGAC	55.0	50.0	57.9	58.1	479 bp	1.0	79.2
psbA-trnH-GUG_p4	TCTAGACCTAGCCGCTATCG	TCACAATCCACTGCCTTGAC	55.0	50.0	57.9	58.1	480 bp	1.0	79.2
psbA-trnH-GUG_p5	CCGTGCTAACCTTGGTATGG	TTTTGTAGTTGGTGGGCGAA	55.0	45.0	58.3	57.9	584 bp	1.0	77.6