Hotspot

trnT-UGU-trnL-UAA

Genome · 61114-61993 · 880 bp

Back to Airampoa

Pi 0.00455

MarkerSeek score 61.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: Genome
length_bp: 880
variable_sites: 4
pi: 0.00455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	953-954 bp	1.0	78.8
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	AGCCAATTAAGTCCGTAGCG	55.0	50.0	58.2	58.1	984-985 bp	1.0	78.6
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	941-942 bp	1.0	77.4
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	972-973 bp	1.0	77.2
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	55.0	50.0	57.9	58.4	952-953 bp	1.0	76.5