Hotspot

psbM-trnD-GUC

Genome · 43059-43932 · 874 bp

Back to Airampoa

Pi 0.00229
MarkerSeek score 60.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
Genome
length_bp
874
variable_sites
2
pi
0.00229
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAAGAGTGCAGTAGC GTTCCCCGGGATTGTAGTTC 45.0 55.0 55.2 58.0 1002 bp 1.0 57.3
psbM-trnD-GUC_p2 ACTAGAATGAAGAGTGCAGTAGC GTTCCCCGGGATTGTAGTTC 43.5 55.0 58.0 58.0 1005 bp 1.0 55.8
psbM-trnD-GUC_p3 AGGAACTAGAATGAAGAGTGCAG GTTCCCCGGGATTGTAGTTC 43.5 55.0 57.9 58.0 1009 bp 1.0 55.2
psbM-trnD-GUC_p4 AGAATGAAGAGTGCAGTAGC AGTTCCCCGGGATTGTAGTT 45.0 50.0 55.2 58.3 1003 bp 1.0 54.9
psbM-trnD-GUC_p5 AGAATGAAGAGTGCAGTAGC TCCCCGGGATTGTAGTTCAA 45.0 50.0 55.2 58.3 1000 bp 1.0 54.9