Hotspot

rpl16

LSC · 76258-77539 · 1282 bp

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Pi 0.00157
MarkerSeek score 60.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1282
variable_sites
2
pi
0.00157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CCCGTTTTAGTTTTAAGGGGT TTCCTGAAATCCCTGAAAAA 42.9 35.0 56.1 52.5 1390-1395 bp 1.0 40.5
rpl16_p2 TCAGAAAGAAAAACAGGGCT TTCCTGAAATCCCTGAAAAA 40.0 35.0 55.1 52.5 1426-1431 bp 1.0 40.4
rpl16_p3 TCAGAAAGAAAAACAGGGCT TTTCCTGAAATCCCTGAAAA 40.0 35.0 55.1 52.5 1427-1432 bp 1.0 40.4
rpl16_p4 TCAGAAAGAAAAACAGGGCT TTTTCCTGAAATCCCTGAAA 40.0 35.0 55.1 52.5 1428-1433 bp 1.0 40.4
rpl16_p5 TCAGAAAGAAAAACAGGGCT TTTTTCCTGAAATCCCTGAA 40.0 35.0 55.1 52.5 1429-1434 bp 1.0 40.4