Hotspot

psaC-ndhE

SSC · 107796-108263 · 468 bp

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Pi 0.00641
MarkerSeek score 59.70
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
468
variable_sites
3
pi
0.00641
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 CTTGGCTTTACATCCCTCCC TTTGTGATAGCCCTTGCAGC 55.0 50.0 57.9 58.8 693-705 bp 1.0 73.3
psaC-ndhE_p2 TGCTTGGCTTTACATCCCTC TTTGTGATAGCCCTTGCAGC 50.0 50.0 57.9 58.8 695-707 bp 1.0 72.7
psaC-ndhE_p3 GTTGGACAAGCTCGTACACA TTTGTGATAGCCCTTGCAGC 50.0 50.0 58.1 58.8 650-662 bp 1.0 72.7
psaC-ndhE_p4 TGTTGGACAAGCTCGTACAC TTTGTGATAGCCCTTGCAGC 50.0 50.0 58.1 58.8 651-663 bp 1.0 72.7
psaC-ndhE_p5 CTGTTGGACAAGCTCGTACA TTTGTGATAGCCCTTGCAGC 50.0 50.0 57.9 58.8 652-664 bp 1.0 72.6