Hotspot

rps15-ndhF

IRb · 100115-100519 · 405 bp

Back to Aira

Pi 0.0272
MarkerSeek score 70.30
Primer available yes
Label rps15-ndhF

Feature Metadata

label_name
rps15-ndhF
feature_type
igs
region
IRb
length_bp
405
variable_sites
11
pi
0.0272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ndhF_p1 CTTTTGGGAAAACGTCGACG ATGTAGGAGGAGGGCGAATC 50.0 55.0 58.0 58.7 587-595 bp 1.0 76.1
rps15-ndhF_p2 CTTTTGGGAAAACGTCGACG AATATGTAGGAGGAGGGCGA 50.0 50.0 58.0 57.3 590-598 bp 1.0 75.8
rps15-ndhF_p3 CTTTTGGGAAAACGTCGACG ATATGTAGGAGGAGGGCGAA 50.0 50.0 58.0 57.3 589-597 bp 1.0 75.8
rps15-ndhF_p4 CTTTTGGGAAAACGTCGACG TATGTAGGAGGAGGGCGAAT 50.0 50.0 58.0 57.3 588-596 bp 1.0 75.8
rps15-ndhF_p5 TCGACGTTTGCTGGCTTATT ATGTAGGAGGAGGGCGAATC 45.0 55.0 58.2 58.7 573-581 bp 1.0 74.7