Hotspot

rpl33-rpl20

LSC · 64213-64903 · 691 bp

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Pi 0.0087
MarkerSeek score 63.90
Primer available yes
Label rpl33-rpl20

Feature Metadata

label_name
rpl33-rpl20
feature_type
igs
region
LSC
length_bp
691
variable_sites
6
pi
0.0087
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rpl20_p1 AGAATCGCCACAATACACCC ATGCTTGCACAAGTAGCTGT 50.0 45.0 58.0 58.1 857-858 bp 1.0 79.6
rpl33-rpl20_p2 CCACAATACACCCGGACAAT ATGCTTGCACAAGTAGCTGT 50.0 45.0 57.9 58.1 850-851 bp 1.0 78.9
rpl33-rpl20_p3 TCGCAAGCATACGACTCATC ATGCTTGCACAAGTAGCTGT 50.0 45.0 58.2 58.1 802-803 bp 1.0 78.6
rpl33-rpl20_p4 CGCAAGCATACGACTCATCA ATGCTTGCACAAGTAGCTGT 50.0 45.0 58.4 58.1 801-802 bp 1.0 76.4
rpl33-rpl20_p5 AGAATCGCCACAATACACCC TGCTTGCACAAGTAGCTGTA 50.0 45.0 58.0 57.5 856-857 bp 1.0 76.0