Hotspot

petA-psbJ

LSC · 59541-60421 · 881 bp

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Pi 0.00684
MarkerSeek score 63.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
881
variable_sites
6
pi
0.00684
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGTTCTTCTTCGCATCCGTT TCCTCTTTGGCTGATAGGTACT 45.0 45.5 57.8 58.0 1072-1076 bp 1.0 63.4
petA-psbJ_p2 TTGTTCTTCTTCGCATCCGT TCCTCTTTGGCTGATAGGTACT 45.0 45.5 57.8 58.0 1073-1077 bp 1.0 63.4
petA-psbJ_p3 TGTTCTTCTTCGCATCCGTT CCTCTTTGGCTGATAGGTACTG 45.0 50.0 57.8 57.9 1071-1075 bp 1.0 62.9
petA-psbJ_p4 TTGTTCTTCTTCGCATCCGT CCTCTTTGGCTGATAGGTACTG 45.0 50.0 57.8 57.9 1072-1076 bp 1.0 62.9
petA-psbJ_p5 TTACGCGTCCAAGGTCTTTT TCCTCTTTGGCTGATAGGTACT 45.0 45.5 57.8 58.0 1091-1095 bp 1.0 62.7