Hotspot

ndhC-trnV-UAC

LSC · 49608-50373 · 766 bp

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Pi 0.00395
MarkerSeek score 61.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
766
variable_sites
3
pi
0.00395
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCCTGAAATCCAAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 58.0 57.9 869-876 bp 1.0 80.1
ndhC-trnV-UAC_p2 TCTCTGGTCCTTCCCTAACC GGTTCGAATCCGTATAGCCC 55.0 55.0 57.8 57.9 899-906 bp 1.0 78.3
ndhC-trnV-UAC_p3 GCCCTGAAATCCAAAATGCC CGGTTCGAATCCGTATAGCC 50.0 55.0 58.0 58.3 870-877 bp 1.0 78.2
ndhC-trnV-UAC_p4 TCTCTGGTCCTTCCCTAACC CGGTTCGAATCCGTATAGCC 55.0 55.0 57.8 58.3 900-907 bp 1.0 76.4
ndhC-trnV-UAC_p5 GCCCTGAAATCCAAAATGCC CGAGAAGGTCTACGGTTCGA 50.0 55.0 58.0 58.9 882-889 bp 1.0 73.3