Hotspot

trnT-GGU-trnE-UUC

LSC · 14900-15444 · 545 bp

Back to Aira

Pi 0.00556
MarkerSeek score 61.70
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
545
variable_sites
3
pi
0.00556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 AGCCCTTTTAACTCAGTGGT AAATACTCCCTACCCCCAGG 45.0 55.0 56.7 57.8 696-701 bp 1.0 68.5
trnT-GGU-trnE-UUC_p2 AGGCATAAGTCATCGGTTCA AAATACTCCCTACCCCCAGG 45.0 55.0 56.6 57.8 659-664 bp 1.0 68.3
trnT-GGU-trnE-UUC_p3 AGCCCTTTTAACTCAGTGGT GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.7 58.3 661-666 bp 1.0 68.1
trnT-GGU-trnE-UUC_p4 AGCCCTTTTAACTCAGTGGT TGTCCTGAACCACTAGACGA 45.0 50.0 56.7 57.7 640-645 bp 1.0 68.0
trnT-GGU-trnE-UUC_p5 AGGCATAAGTCATCGGTTCA GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.6 58.3 624-629 bp 1.0 67.9