Hotspot

trnS-GCU-psbD

LSC · 7671-8663 · 993 bp

Back to Aira

Pi 0.00202
MarkerSeek score 60.00
Primer available yes
Label trnS-GCU-psbD

Feature Metadata

label_name
trnS-GCU-psbD
feature_type
igs
region
LSC
length_bp
993
variable_sites
2
pi
0.00202
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbD_p1 ATTAGCAATCCGCCGCTTTA GCCGGACCATCCTACAAAAA 45.0 50.0 58.0 58.2 1138 bp 1.0 78.5
trnS-GCU-psbD_p2 ATTAGCAATCCGCCGCTTTA CGGTCTCTTCGTAACCAGTC 45.0 55.0 58.0 57.7 1113 bp 1.0 77.8
trnS-GCU-psbD_p3 GAGCGGAAAGAGAGGGATTC GCCGGACCATCCTACAAAAA 55.0 50.0 57.8 58.2 1189 bp 1.0 77.0
trnS-GCU-psbD_p4 ATTAGCAATCCGCCGCTTTA ACGGTCTCTTCGTAACCAGT 45.0 50.0 58.0 58.4 1114 bp 1.0 76.8
trnS-GCU-psbD_p5 GAGCGGAAAGAGAGGGATTC CGGTCTCTTCGTAACCAGTC 55.0 55.0 57.8 57.7 1164 bp 1.0 76.3