Hotspot

trnK-UUU-rps16

LSC · 3969-4561 · 593 bp

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Pi 0
MarkerSeek score 61.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
593
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TGGACGAATTTGTTGCTCCA GCTCAACCGACAAGAACTGT 45.0 50.0 58.0 58.4 741-757 bp 1.0 78.2
trnK-UUU-rps16_p2 TGGACGAATTTGTTGCTCCA GTGCTCAACCGACAAGAACT 45.0 50.0 58.0 58.4 743-759 bp 1.0 78.2
trnK-UUU-rps16_p3 TGGACGAATTTGTTGCTCCA TGCTCAACCGACAAGAACTG 45.0 50.0 58.0 58.4 742-758 bp 1.0 78.2
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG GCTCAACCGACAAGAACTGT 50.0 50.0 58.6 58.4 700-716 bp 1.0 73.9
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG GTGCTCAACCGACAAGAACT 50.0 50.0 58.6 58.4 702-718 bp 1.0 73.9