Hotspot

ndhF-rpl32

SSC · 113281-113577 · 297 bp

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Pi 0.0204
MarkerSeek score 50.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
297
variable_sites
6
pi
0.0204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AATCGGTCGTGTGAACCAAA TTCGGGCTATGTCAATTCCG 45.0 50.0 58.0 58.1 1002-1009 bp 1.0 80.2
ndhF-rpl32_p2 ATCGGTCGTGTGAACCAAAA TTCGGGCTATGTCAATTCCG 45.0 50.0 58.0 58.1 1001-1008 bp 1.0 80.2
ndhF-rpl32_p3 CATACGTGGAGGGGGAATTG TTCGGGCTATGTCAATTCCG 55.0 50.0 58.0 58.1 1238-1245 bp 1.0 80.1
ndhF-rpl32_p4 ATCGGTCGTGTGAACCAAAA AGTTCCCCCGATACAGTTGA 45.0 50.0 58.0 58.1 1401-1409 bp 1.0 80.0
ndhF-rpl32_p5 CATACGTGGAGGGGGAATTG AGTTCCCCCGATACAGTTGA 55.0 50.0 58.0 58.1 1638-1646 bp 1.0 79.9