Hotspot

ycf3-trnS-GGA

LSC · 44196-44815 · 620 bp

Back to Aiphanes

Pi 0.00645
MarkerSeek score 62.50
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
620
variable_sites
4
pi
0.00645
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 783 bp 1.0 67.0
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 784 bp 1.0 67.0
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 804 bp 1.0 66.5
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 805 bp 1.0 66.5
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTGAA 45.0 45.0 56.4 57.8 754 bp 1.0 66.2