Hotspot

trnT-GGU-psbD

LSC · 31687-32667 · 981 bp

Back to Aiphanes

Pi 0.00715

MarkerSeek score 62.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 981
variable_sites: 7
pi: 0.00715
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1148-1150 bp	1.0	77.9
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1143-1145 bp	1.0	77.8
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1132-1134 bp	1.0	77.8
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	50.0	55.0	57.9	57.7	1127-1129 bp	1.0	77.8
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	GCCAGACCAGCCTACAAAAA	50.0	50.0	58.1	58.4	1161-1163 bp	1.0	76.8