Hotspot

trnE-UUC-trnT-GGU

LSC · 31331-31614 · 284 bp

Back to Aiphanes

Pi 0.0246

MarkerSeek score 57.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 7
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	387 bp	1.0	79.3
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	366 bp	1.0	79.2
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	352 bp	1.0	78.7
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	331 bp	1.0	78.6
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	384 bp	1.0	78.1