Hotspot

ycf2

LSC · 89711-96703 · 6993 bp

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Pi 0.00257
MarkerSeek score 41.20
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
LSC
length_bp
6993
variable_sites
18
pi
0.00257
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 CAGCCCTTTGTCTCTGTGTT TATTCCATCGATCCCCACGA 50.0 50.0 58.0 58.0 598 bp 1.0 82.6
ycf2_p2 GTTCTTGCAGAGTGGAACCA TATTCCATCGATCCCCACGA 50.0 50.0 58.0 58.0 722 bp 1.0 82.0
ycf2_p3 CAGCCCTTTGTCTCTGTGTT AAATGGTAGACACGCGAGAC 50.0 50.0 58.0 58.0 762 bp 1.0 81.9
ycf2_p4 CAGCCCTTTGTCTCTGTGTT TGAAATGGTAGACACGCGAG 50.0 50.0 58.0 58.0 764 bp 1.0 81.9
ycf2_p5 GTTCTTGCAGAGTGGAACCA AAATGGTAGACACGCGAGAC 50.0 50.0 58.0 58.0 886 bp 1.0 81.6